Chromogenic and fluorogenic substrates

Chromogenic substrates
Fluorogenic substrates

S-2238

Is the chromogenic substrate D-Phenylalanyl-L-Pipecolyl-p-nitroaniline dihydrochloride that is specific for thrombin and antithrombin. Its amino acid sequence increases its affinity and rates of cleavage > 1000 fold. The method for the determination of activity is based on the difference in absorbance (optical density) between the p-nitroaniline (pNA) formed and the original substrate. The rate of pNA formation, i.e. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity and its conveniently determined with a spectrophotometer.

Typical properties: Formula H-D-Phe-Pip-Arg-pNA
Empirical formula C27H36N8O· 2 HCl
Molecular weight 625.6 g·mol-1
CAS number 113711-77-6
Solubility > 10 mmol·L-1
e316 nm 1.27 x 104 mol-1·L·cm-1
Package: 25 mg
Art. No.: 930100
Storage conditions: Shipped at ambient temperature. Store at -20 °C.

 

S-2238

 

Order information

S-2765

Is the chromogenic substrate N-α-Benzoyloxycarbonyl-D-arginyl-L-Glycyl-L-Arginine-p-nitroaniline dihydrochloride that is specific for Factor Xa. Its amino acid sequence increases its affinity and rates of cleavage > 1000 fold. The method for the determination of activity is based on the difference in absorbance (optical density) between the p-nitroaniline (pNA) formed and the original substrate. The rate of pNA formation, i.e. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity and its conveniently determined with a spectrophotometer.

Typical properties: Formula N-α-Z-D-Arg-Gly-Arg-pNA
Empirical formula  C28H39N11O7 . 2 HCl
Molecular weight 714.6 g·mol-1
CAS number 115388-96-0
Solubility > 40 mmol·L-1 in H20; > 10 mol·l-1 in Tris Buffer pH 8.3, I 0.25
e316 nm 1.27 x 104 mol-1·L·cm-1
Package: 25 mg
Art. No.: 930110
Storage conditions: Shipped at ambient temperature. Store at -20 °C.

 

S-2765

 

Order information

Z-Gly-Gly-Arg-AMC

Is suitable for the quantitative direct fluorescent assay of urokinase and is also useful as a substrate for plasminogen activators.

Z-GGR-AMC is suitable for the quantitative direct fluorometric assay of urokinase and tissue-type plasminogen activator as well as for assaying trypsin and thrombin.
Z-GGR-AMC is used as thrombin substrate in the calibrated automated thrombogram (CAT), an automated test developed by Hemker et al., for determining hyper- and hypocoagulability.
Z-GGR-AMC can also be used for determining the tryptic activity of proteasomes.

Typical properties: Formula Z-Gly-Gly-Arg-AMC
Empirical formula C28H33N7O7 · HCl
Molecular weight 616.07 g.mol-1
CAS number: 102601-58-1
Solubility DMSO
 
White to off-white lyophilized powder.
Package: 25 mg
Art. No.: 930200
Storage conditions: Shipped at ambient temperature. Store at – 20 °C.

AMC-Substrate

 

Order information

  • Downloads

    Product profile sheets

     

    MSDS